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DNA Barcode Assay

Cell Line Characterization - DNA barcode assay

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CO1 Barcode Assay

The cytochrome c oxidase subunit 1 (CO1) gene is an ideal target for species discrimination as this gene is conserved across higher eukaryotes, including insects, fish, birds and mammals. CO1 is located on mitochondrial DNA, meaning it has a relatively fast mutation rate that aligns with speciation timescales.

An approximately 650bp region of CO1 known as the barcode region is amplified with degenerate primers followed by DNA sequence analysis by the Sanger method. For insect species a smaller region of approximately 450bp is targeted on the CO1 gene.

The obtained DNA sequence is compared against a known standard for species confirmation.


BioReliance offers two cell identity test services based on CO1 barcode methodology:

CO1 Barcode Assay for Cell Line Identification

Degenerate primers were designed, optimized and validated to target the ~650bp CO1 barcode region. Current validated reference sequences offered are:

  • Human (e.g. HEK293)
  • Mouse (Mus musculus e.g. SP2/0)
  • Chinese Hamster (e.g. (CHO)
  • Syrian Hamster (e.g. BHK)
  • African Green Monkey (e.g. Vero)

CO1 Barcode Assay for Insect Cell Line Identification


Degenerate primers were designed, optimized and validated to target the ~450bp CO1 barcode region for insects. Current validated reference sequences offered are:

  • Spodoptera frugiperda (e.g. Sf9)

Additional species coverage will be added shortly – please contact BioReliance to discuss your requirements.

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