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Back to Retrovirology
Cocultivation and amplification assays with Mink lung, human RD, human A549, human MRC-5, human WI-38, human Raji, human HUT 78, human primary peripheral blood lymphocytes, human 293, murine Mus dunni, murine SC-1, chick embryo fibroblasts, quail QT6, turkey embryo fibroblasts.
Cell lines used in the manufacture of biopharmaceuticals are derived from a number of mammalian species and are known to contain a variety of retroviruses. In some cases, those retroviruses exist solely as proviral sequences that are not expressed; are expressed as non-infectious particles (e.g., in CHO cells); or are expressed only at very low levels. Cocultivation of cells at the limit of the in vitro cell age (end of production cells) or of cells from a master cell bank with susceptible cell lines allows for amplification of these retroviruses and enhances the ability to detect their presence. Either live cells or cryopreserved cells can be submitted for testing.
Amplification assays are performed by using the same cell lines and in a manner analogous to the cocultivation assays. However, instead of live cells, the amplification assays use cell-free inocula, such as vaccines or cell-free culture supernatant.
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