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MAP-IT, Molecular Antigen PCR - Identification Tests.
A fast alternative to the in vivo MAP assay for laboratory animal diagnostics

The Molecular Antigen PCR-Identification Test (MAP-IT) is a combination of gel-based PCR and real time PCR assays for detecting viral agents that are currently tested by the in vivo assay. Instead of injecting test materials into the animals and waiting for the immune response from the mice, the molecular based assay directly detects the presence of any viral nucleic acid in the samples. The detection of any viral nucleic acid indicates that there may be viral contamination present.

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Laboratory Animal Diagnostic Services at BioReliance can provide a variety of reagents to our clients.

Reagents

West Nile Virus Reagents
LADS Reference Reagents

West Nile Virus Research Agents

Several monoclonal antibodies against West Nile Virus Protein E are available, supplied in a 0.2 µl filtered solution of PBS with 0.01% Thimerosal. All are suitable for ELISA and Western Blot applications. See table for additional characteristics. Equine and Avian Assay Protocols are available upon request.

Description	Unit	Catalog No.
West Nile Virus Monoclonal Antibody 7H2	100 µg	81-002
West Nile Virus Monoclonal Antibody 5H10	100 µg	81-003
West Nile Virus Monoclonal Antibody 3A3	100 µg	81-004
West Nile Virus Monoclonal Antibody 5C5	100 µg	81-005
West Nile Virus Monoclonal Antibody 3D9	100 µg	81-006
West Nile Virus Antigen for Avian or Equine IgG (irradiated), plus Control	500 tests	81-010
West Nile Virus Antigen for Equine IgM (irradiated), plus Control	500 tests	81-011
West Nile Virus Positive and Negative Antibody for Equine IgG	500 tests	81-012
West Nile Virus Positive and Negative Antibody for Equine IgM	500 tests	81-013
West Nile Virus High Positive, Low Positive and Negative Antibody Set for Avian IgG	20 tests	81-014
West Nile Virus Positive and Negative Antibody for Rabbit IHC	50 tests	81-015
Anti-Equine IgM	500 tests	80-565
Anti-Equine IgG HRP-Labeled Conjugate	500 tests	80-562
Anti-Mouse IgM HRP-Labeled Conjugate	500 tests	80-559
Peroxidase Substrate	500 tests	80-566

West Nile Virus Monoclonal Antibody Characteristics

Monoclonal Antibody	IgG Class	Neutral Titer, PFU	ELISA TIter	Cross-Reactivity with JEV	Recommendations
3A3	IgG2a	>10,240	1.6x10⁵	-	Best for IFA
5C5	IgG2a	1,024	1.6x10⁵	+/-
5H10	IgG2a	>10,240	3.2x10⁵	-	IHC dilution 1:400
3D9	IgG1	<40	2.5x10⁴	++
7H2	IgG2a	>10,240	3.2x10⁵	-	IHC dilution 1:400

West Nile Virus Detection Assay Components

Assay	Antigen	Antibody	Capture Antibody	Detection Antibody	Conjugate Antibody	Substrate
Avian IgG	81-010	81-014	-	-	80-557	80-566
Equine IgG	81-010	81-012	81-002	80-562	80-562	80-566
Equine IgM	81-010	81-013	80-565	81-002	80-559	80-566

LADS Reference Reagents

LADS Reference Reagents are polyclonal antibodies originally developed in collaboration with the American Committee on Laboratory Animal Disease (ACLAD). These antisera are suitable for use in ELISA and Western Blot applications. Each are supplied pre-diluted 1:5 in sterile water. Store antisera frozen, at -20°C or below.

Antisera Prepared from Mice

Antisera To:	Unit	Catalog No.
Adenovirus, Strain FL (M.Ad-1)	25 tests	80-361
Adenovirus, Strain K-87 (M.Ad-2)	25 tests	80-362
C. piliforme (Tyzzer's)	25 tests	80-351
Ectromelia Virus (Ectro)	25 tests	80-353
Epizootic Diarrhea of Infant Mice (EDIM)	25 tests	80-354
K Virus	25 tests	80-358
Lymphocytic Choriomeningitis Virus (LCMV)	25 tests	80-360
Minute Virus of Mice (MVM)	25 tests	80-367
Mouse Cytomegolovirus (MCMV)	25 tests	80-364
Mouse Hepatitis Virus (MHV)	25 tests	80-365
M. arthritidis	25 tests	80-368
M. muris	25 tests	80-370
M. pulmonis	25 tests	80-372
Mouse Thymic Virus (MTV)	25 tests	80-366
Polyoma Virus	25 tests	80-374
Pneumonia Virus of Mice (PVM)	25 tests	80-375
Reovirus (Reo)	25 tests	80-378
Sendai Virus	25 tests	80-379
Theiler's Mouse Encephalomyelitis (TMEV/GDVII)	25 tests	80-355
Negative Control	25 tests	80-383

Antisera Prepared from Rats

Antisera To:	Unit	Catalog No.
C.piliforme (Tyzzer's)	25 tests	80-352
CAR Bacillus (CARB)	25 tests	80-381
H-1 Virus	25 tests	80-357
Hantaan Virus	25 tests	80-356
Kilham Rat Virus (KRV)	25 tests	80-359
M. arthritidis	25 tests	80-369
M. muris	25 tests	80-371
M. pulmonis	25 tests	80-373
Pneumonia Virus of Mice (PVM)	25 tests	80-376
Rat Coronavirus/Sialoda-cryoadenitis Virus (RCV/SDA)	25 tests	80-377
Reovirus (Reo)	25 tests	80-382
Sendai Virus	25 tests	80-380
Negative Control	25 tests	80-384

for further information and enquiries please contact: info@bioreliance.com