Cell Line Authentication

We offer a variety of services and assays for determining cell line identity and authenticating your cell lines used for production and drug discovery research.

In general, we recommend cell lines should be authenticated as newly established or acquired cell lines, before freezing, once every two months of active culture growth, if cell performance is inconsistent with expected or historical results, or before publication of results.

 

BioReliance offers the following cell line authentication and identity testing services:

  • CO1 barcode assay
  • DNA analysis (DNA fingerprinting, DNA sequencing)
  • Karyotyping
  • Random Amplification of Polymorphic DNA (RAPD)

 

Custom authentication services also are available.  Contact BioReliance and our research team will be happy to assist you in planning your cell line characterization studies. Below you will find more technical information about our cell authentication services.

**March 2015 – Isoenzyme analysis has been withdrawn until further notice.
For more details click here.


CO1 Barcode Assay

DNA sequence for the cytochrome c oxidase subunit 1 gene (CO1) barcode region is determined by the Sanger method for confirmation of the species of origin for the cell type.

Click here for more details on the CO1 barcode assay

Contact BioReliance and our research team will be happy to assist you in planning your cell line authentication studies. 

 

Karyotyping

BioReliance scientists have performed many karyotyping (banded chromosome analysis) services for cell line authentication studies.  Results can confirm your sample’s cell line identity and species of origin. 

Standard karyotyping analysis includes, but isn’t limited to:

  • Metaphase nuclei harvest and chromosome count (to give the modal diploid chromosome number, frequency distribution and polyploidy levels)
  • Chromosomal abnormality quantification
  • Metaphases G-banded and, where feasible, karyotypes are constructed

  

The BioReliance team has extensive experience in karyotyping cell lines derived from multiple species used in the production of biologicals and drug discovery such as human (including stem cells), monkey, mouse, rat, rabbit, dog, hamster, and insect (baculovirus). 

Karyotyping studies also can be customized to your study needs.  Please contact us for more information.

 

DNA Analysis (DNA fingerprinting, genotyping)

DNA analysis is used as an alternative, or performed in addition to, isoenzyme and karyotyping authentication studies for certain types of cell lines. By interrogating endonuclease digested genomic DNA with probes to hypervariable regions, cell line identity and/or cross contamination can be determined.

BioReliance CRO provides comprehensive DNA analysis techniques including:

  • AFLP (Amplified Fragment Length Polymorphism) detection
  • RFLP (Restriction Fragment Length Polymorphism) identification of genomic DNA
  • SSR (Simple Sequence Repeat)
  • EST (Expressed Sequence Tag)
  • SNP (Single Nucleotide Polymorphism)

 

RAPD Assays

Random amplified polymorphic DNA (RAPD) analysis allows detection of genomic polymorphisms using nanogram amounts of DNA.  The assay uses single short oligonucleotide primers and PCR to generate strain specific products that are compared to standards for the target cell line for authentication.  This method can be used on both prokaryotic and eukaryotic cells. 

RAPD assay design for the authentication of mammalian, microbial and other cell lines has to be species-specific.  Further, the results can vary depending on the history of the cell line and the type of media used for cell growth. 

For these reasons, we suggest consulting with a BioReliance expert who will help you determine the best methods for using RAPD for cell line authentication that are suited for your needs. Click here to contact us.

Contact Us: +03-6758-3610

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