Identity Assays
Cell and Virus Characterization - Assays and Services
BioReliance offers the following services and assays for your Cell and Virus Characterization:
Isoenzyme Analysis
Unique native cell enzyme patterns may be compared quantitatively between different cell lines since enzyme activities are expressed by different, though related species of molecular origin. Cellular isoenzymes can be separated by electrophoresis and the resulting banding patterns obtained used to identify species of origin.
Karyology Services
For the confirmation of the species of origin and identity of cell lines, metaphase nuclei are harvested and the chromosomes counted to give the modal diploid chromosome number, frequency distribution and polyploidy levels. Chromosomal abnormalities are quantified. Metaphases are also G-banded and, where feasible, karyotypes are constructed. The Cell Biology group has extensive experience in the karyology of cell lines derived from many species such as human (including stem cells), monkey, mouse, rat, rabbit, dog, cat, mink, cow, hamster, chicken, duck and insect.
DNA Fingerprint Assays
By interrogating endonuclease digested genomic DNA with probes to hypervariable regions, cell line identity and/or cross contamination can be determined. DNA fingerprinting is used as an alternative or performed in addition to isoenzyme and karyology studies for certain types of cell lines.
RAPD Assays
Random amplified polymorphic DNA (RAPD) analysis allows detection of genomic polymorphisms using nanogram amounts of DNA. The assay uses single short oligonucleotide primers and polymerase chain reaction to generate strain specific products. This method can be used on both prokaryotic and eukaryotic cells.
